Figure 3.

SIRT1 liver specific knockout leads to increased expression of lipid metabolism genes. (A) The expression of genes involved in glycolysis, β-oxidation, esterificaiton and fat uptake is not changed in the livers of Sirt1^LKO male mice at 2 months of age (n≥6). (B) Sirt1^LKOmice display elevated expression of FAS, ACC1 and ELOVL6 at 2 months (n≥6). *p<0.05. (C) At 6 months of age, the expression of FAS, ACC1, ELOVL6 and SCD1 remains increased in Sirt1^LKOmice (n≥6). *p<0.05. (D-E) The expression of SREBP-1c is down regulated in Sirt1^LKOmice at 2 months of age at both mRNA level (C) and protein level (D). (F) In vitro, deletion of SIRT1 (compare last two bars) or knocking down SIRT1 by siRNA (the first two bars) reduces mRNA level of SREBP-1c, while over expressing SIRT1 (middle two bars) increases SREBP-1c mRNA level. * p<0.01