Figure 5.

Effect of cNOS inhibitor, L-NAME (LN), and ERK1/2 inhibitor, PD98059 (PD), on ghrelin (Gh)-induced cPLA₂ S-nitrosylation in sublingual salivary gland acinar cells exposed to ethanol (Et). The cells were treated with Gh (0.7 μg/ml) or L-NAME (400 μM) + Gh or PD98059 (30 μM)+Gh and incubated for 2 h in the presence of Et. A portion of the cell lysate was processed by biotin switch procedure for protein S-nitrosylation and, along with the reminder of the lysates, subjected to SDS-PAGE, transferred to nitrocellulose and probed with phosphorylation specific cPLA₂ (p-cPLA₂) antibody, and reprobed with antitotal cPLA₂ antibody. The immunoblots shown are representative of three experiments.