Table 3.
drp2 Allele | Line | Insert Position | Left Primer | Right Primer |
drp2a-1 | SALK_071036 | 2246 | 1164 | 1165 |
drp2a-3 | SALK_011319 | 3979 | 1171 | 1172 |
drp2a-4 | SALK_018859 | 1374 | 858 | 1165 |
drp2b-1 | SALK_003049 | 7145 | 865 | 1056 |
drp2b-2 | SALK_134887 | 1120 | 1161 | 1160 |
drp2b-3 | SALK_124686 | 6396 | 861 | 865 |
drp2b-4 | WISCDSLOX_256E05 | 255 | 1162 | 1163 |
drp2b-5 | SALK_041330 | 1305 | 1160 | 1161 |
The T-DNA insert was detected by PCR amplification of total genomic DNA preps using a T-DNA primer (774 for line drp2b-4 and 926 for all other lines) in combination with the left primer; the absence of the insert (to distinguish heterozygous from homozygous plants) was detected by amplification using the left and right primers. The exact position of the insert (number of bases 5′ of ATG in genomic sequence) was determined by direct sequencing of the genotyping PCR reaction. Primer sequences are listed in Supplemental Table 2 online.