Table 3.

Genotyping of drp2 Insertional Alleles

drp2 Allele	Line	Insert Position	Left Primer	Right Primer
drp2a-1	SALK_071036	2246	1164	1165
drp2a-3	SALK_011319	3979	1171	1172
drp2a-4	SALK_018859	1374	858	1165
drp2b-1	SALK_003049	7145	865	1056
drp2b-2	SALK_134887	1120	1161	1160
drp2b-3	SALK_124686	6396	861	865
drp2b-4	WISCDSLOX_256E05	255	1162	1163
drp2b-5	SALK_041330	1305	1160	1161

The T-DNA insert was detected by PCR amplification of total genomic DNA preps using a T-DNA primer (774 for line drp2b-4 and 926 for all other lines) in combination with the left primer; the absence of the insert (to distinguish heterozygous from homozygous plants) was detected by amplification using the left and right primers. The exact position of the insert (number of bases 5′ of ATG in genomic sequence) was determined by direct sequencing of the genotyping PCR reaction. Primer sequences are listed in Supplemental Table 2 online.