Figure 4.

MAT3/RB Interacts with E2F1-DP1 and Requires the C Terminus of E2F1 for Interaction.

(A) Untagged E2F1 or E2F1Δ382 was tested for their ability to bridge MAT3-AD and DP1-DBD or DP1Δ340-DBD in the Y2H assay. Y2H constructs are labeled the same as in Figure 3A. Additional controls are in Supplemental Figure 7 online.

(B) Immunoblots to detect 3XHA-MAT3 and DP1. Light-dark regime and cell cycle stage/time are depicted above and below each blot. Lysates prepared from equal numbers of mat3-4::3XHA-MAT3 cells were loaded in each lane. Top and middle panels are immunoblots probed with anti-HA and anti-DP1. Bottom panel is a Coomassie blue–stained gel to show total protein loaded on the basis of equal cell number per lane. Note that the 16-h time point was loaded based on mother cell number. Below each gel lane is a micrograph of cells from the corresponding time point. Bar = 10 μm.