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. 2010 Oct 26;22(10):3331–3347. doi: 10.1105/tpc.110.076067

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© 2010 American Society of Plant Biologists

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Figure 7. — MAT3/RB Binds to Chromatin throughout the Cell Cycle and Requires DP1 for Chromatin Binding.

(A) Immunoblot of IP inputs (In), unbound supernatant fraction (S), and anti-HA IPs (IP) from mat3-4::3XHA-MAT3 DP1 and mat3-4::3X-HA-MAT3 dp1-1 probed with antibodies raised to HA (HA-MAT3), DP1, and Histone H3 (H3).

(B) Immunoblot of anti-HA IPs (lanes 1 and 2) and IP inputs (lanes 3 and 4) from G1- and S/M-phase synchronized mat3-4::3XHA-MAT3 lysates probed with antibodies against HA (to detect HA-MAT3), DP1, Histone H3 (H3), Histone H3S10P (H3S10P), and α-tubulin.