Table 1.

Measurement of Lignin in Stems of Transgenic Perennial Ryegrass hpOMT1 and hpCCR1 Lines at the R1 Stage Using the Acetyl Bromide Method and GC-MS

	Glasshouse							Field Trial
Line	AcBr Lignina	G Ligninb	S Ligninb	H Ligninb	S/G	S/H	IVVDMD	AcBr Lignina	G Ligninb	S Ligninb	H Ligninb	S/G	S/H	IVVDMD
Control	16.94 ± 0.7	24.6 ± 1.8	19.0 ± 1.5	0.81 ± 0.04	0.77	23.4	61.0	13.3 ± 2.8	12.1 ± 3.5	10.8 ± 0.1	0.49 ± 0.01	0.89	22.0	75.65
hpOMT1-2	16.04 ± 1.2	24.5 ± 1.6	21.5 ± 1.8	0.74 ± 0.04	0.88	29.4
hpOMT1-3	14.24 ± 1.3	19.6 ± 2.5	12.5 ± 1.8	0.79 ± 0.04	0.63	15.8	67.1	12.0 ± 0.8	8.6 ± 0.8	7.9 ± 0.1	0.43 ± 0.01	0.91	18.3	82.60
hpOMT1-6	14.51 ± 0.6	25.5 ± 1.5	20.5 ± 1.9	0.67 ± 0.02	0.80	30.5
hpOMT1-8	15.72 ± 0.8	21.4 ± 1.5	13.4 ± 2.5	0.78 ± 0.04	0.62	17.1	67.6
hpOMT1-10	15.84 ± 0.4	26.7 ± 1.8	21.5 ± 1.2	0.69 ± 0.05	0.80	31.0
hpOMT1-11	16.21 ± 0.5	27.9 ± 1.0	21.4 ± 1.4	0.83 ± 0.04	0.76	25.7
hpOMT1-12	16.09 ± 0.4	24.3 ± 1.2	20.5 ± 1.8	0.80 ± 0.01	0.84	25.6
hpOMT1-13	15.64 ± 0.2	21.9 ± 1.4	15.9 ± 1.8	0.79 ± 0.04	0.72	20.1	63.5
hpCCR1-1	11.64 ± 0.27	14.3 ± 1.6	10.3 ± 1.4	0.42 ± 0.09	0.70	24.5	68.5	8.5 ± 1.6	3.0 ± 0.9	2.80 ± 0.4	0.17 ± 0.01	0.96	16.4	87.43
hpCCR1-2	16.60 ± 0.17	24.4 ± 2.9	23.8 ± 2.1	0.79 ± 0.12	0.97	29.8
hpCCR1-3	12.64 ± 0.20	17.5 ± 2.6	13.9 ± 1.6	0.57 ± 0.04	0.79	24.3	68.3
hpCCR1-4	14.64 ± 0.17	18.1 ± 1.4	15.1 ± 1.7	0.71 ± 0.11	0.83	21.3
hpCCR1-5	16.04 ± 0.21	23.1 ± 2.4	20.8 ± 2.4	0.73 ± 0.15	0.9	28.4
hpCCR1-6	13.04 ± 0.29	17.9 ± 1.7	14.0 ± 1.3	0.55 ± 0.09	0.78	24.4	67.9

Lines shown in bold had the greatest reduction in the level of CCR1 or OMT1 gene expression.

^aSoluble lignin, percentage of dry cell walls.

^bmg/g dry cell walls.