Table 2.

Measurement of Lignin in the 3rd Internode of Perennial Ryegrass hpOMT1 and hpCCR1 Lines at the R1 Stage Using the Acetyl Bromide Method and GC-MS

Linea	AcBrb Lignin	G Ligninb	S Ligninc	H Ligninc	S/G	S/H
Control	11.29 ± 1.3	17.8 ± 2.8	11.2 ± 1.5	0.32 ± 0.1	0.68	35.0
hpOMT1-2	11.49 ± 1.2	18.8 ± 2.6	13.1 ± 1.8	0.31 ± 0.1	0.63	42.2
hpOMT1-3	6.90 ± 0.3	12.1 ± 0.8	5.4 ± 0.8	0.36 ± 0.1	0.44	15.0
hpOMT1-6	12.05 ± 0.8	20.0 ± 1.9	12.9 ± 1.9	0.30 ± 0.1	0.71	43.0
hpOMT1-8	9.33 ± 0.7	13.3 ± 2.5	7.7 ± 1.5	0.29 ± 0.1	0.57	26.5
hpOMT1-10	10.56 ± 0.8	20.6 ± 2.8	13.4 ± 1.2	0.35 ± 0.1	0.65	38.2
hpOMT1-11	11.60 ± 0.8	20.1 ± 2.1	12.6 ± 1.6	0.25 ± 0.1	0.62	50.1
hpOMT1-12	10.73 ± 0.9	19.5 ± 2.2	12.4 ± 1.7	0.27 ± 0.1	0.63	45.9
hpOMT1-13	9.68 ± 0.7	13.8 ± 1.6	7.3 ± 1.6	0.30 ± 0.1	0.52	24.3
hpCCR1-1	4.58 ± 0.7	9.5 ± 1.6	5.4 ± 1.9	0.20 ± 0.1	0.56	27.1
hpCCR1-2	10.08 ± 1.7	18.9 ± 2.6	12.2 ± 1.6	0.30 ± 0.1	0.64	40.0
hpCCR1-3	7.01 ± 1.8	13.6 ± 1.6	7.4 ± 2.6	0.23 ± 0.1	0.54	32.1
hpCCR1-4	9.78 ± 0.6	16.9 ± 3.0	11.2 ± 3.6	0.29 ± 0.1	0.66	38.6
hpCCR1-5	11.60 ± 2.7	17.1 ± 3.2	10.2 ± 2.9	0.26 ± 0.1	0.59	39.3
hpCCR1-6	7.98 ± 0.3	14.8 ± 1.9	9.1 ± 2.6	0.25 ± 0.1	0.61	36.4

Lines shown in bold had the greatest reduction in the level of CCR1 or OMT1 gene expression.

^aPlants were grown under glasshouse conditions.

^bSoluble lignin, percentage of dry cell walls.

^cmg/g dry cell walls.