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. 2010 Oct 1;22(10):3280–3294. doi: 10.1105/tpc.109.070821

Figure 4.

Figure 4.

Accumulation of Glutelins, BEI, and RA16 in Developing Seeds.

(A) Accumulation of glutelins in developing seeds of the wild type (Kinmaze), flo2 mutant (EM37), VC, and CL. Each lane contains proteins from one-hundredth of the total glutelin fraction, which was extracted from the mixture of 10 rice grains of each line. Proteins are shown by Coomassie blue staining after separation on SDS-PAGE. Arrows indicate 51-kD glutelin precursor, 30- to 36-kD α-subunits of glutelins, and 19- to 22-kD β-subunits of glutelins, respectively. Size markers are shown on the left.

(B) and (C) Accumulation of BEI (B) and RA16 (C) in developing seeds 10 DAF of the wild type, flo2 mutant, VC (three independent lines), and CL (five independent lines) is shown by protein gel blot analysis using antiserum raised against BEI and RA16, respectively. The RA16 antiserum detects highly conserved 14- to 16-kD allergenic proteins with slightly different molecular mass. Each lane contains 1 μg of total protein, which was separated by SDS-PAGE.