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. 2010 Oct 1;22(10):3280–3294. doi: 10.1105/tpc.109.070821

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© 2010 American Society of Plant Biologists

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Figure 5. — Positional Cloning and Structure of Rice FLO2.

(A) Fine mapping of the FLO2 locus. The region of the FLO2 locus was mapped to a 354-kb region by published EST/SSR markers (RM3814 and RM3335-1), then narrowed to a 37-kb region by newly created markers (218042 and 218787) in chromosome 4 (Chr. 4), which contained four predicted genes shown by the gene names registered in the database. Markers used for the mapping are shown in the figure and are detailed in Supplemental Table 2 online.

(B) Exon/intron structure of FLO2. The FLO2 gene is composed of 23 exons (filled box) with 22 introns, including three TPR motifs (open box) in the middle. Eight mutants showing the flo2 phenotype contained one base substitution in the FLO2 gene that generated a premature stop codon (EM36, EM37, EM139, EM280, and EM554), a presumable splicing site (EM373 and EM624), or an amino acid change (EM756).