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. 2010 Oct 1;22(10):3280–3294. doi: 10.1105/tpc.109.070821

Figure 7.

Figure 7.

Interaction of LEA and bHLH with FLO2.

(A) and (B) Y2H analysis of FLO2 with a LEA protein (accession number AK061818) (A) and a bHLH protein (accession number AK070651) (B). LEA, bHLH, FLO2(N), and FLO2(C) indicate yeast cells containing plasmids for expression of the LEA, bHLH, FLO2(1–898), and FLO2(900–1720), respectively. EV(B) and EV(P) show those containing the empty bait and prey vectors instead of the corresponding genes, respectively.

(C) and (D) Reciprocal swapping using LEA and FLO2 (C) and bHLH and FLO2 (D). FLO2(W) and FLO2(M) indicate yeast cells containing plasmids for expression of the full-length FLO2 and FLO2(535–1189), respectively. These cells were grown on agar plates lacking Trp, Leu, and His in the medium.