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Kinetic Characterization of Plant AAHs With Respect to the Cofactor
| Enzyme | H4BPt Cofactor |
10-CHO-THF Cofactor |
||
| Km (μM) | Kcat (s−1) | Km (μM) | Kcat (s−1) | |
| P. taeda | 281 ± 14 | 0.132 ± 0.002 | 293 ± 13 | 2.30 ± 0.01 |
| P. patens | 203 ± 6 | 1.16 ± 0.01 | 950 ± 34 | 6.89 ± 0.19 |
The Phe concentration was 20 mM. Values are means and se of three replicates.
