Characterization of the Complemented cys-c1Mutant Lines.
(A) β-Cyanoalananine synthase activity of the complemented cys-c1:P35S-CYSC1.1 and cys-c1:P35S-CYSC1.24 lines was quantified in root tissue of 4-week-old plants by measuring the formation of sulfide. One unit of activity corresponds to the formation of 1 nmol min−1 of sulfide. Values are means ± sd of three independent experiments.
(B) Root phenotype of the cys-c1 and complemented mutant lines. Bright-field image of 2-week-old seedlings growing on MS medium. a, The wild type; b, cys-c1 mutant line; c, complemented cys-c1:P35S-CYSC1.1 mutant line; d, complemented cys-c1:P35S-CYSC1.24 mutant line.