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. 2010 Oct 22;22(10):3474–3488. doi: 10.1105/tpc.110.075861

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© 2010 American Society of Plant Biologists

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Figure 6. — Analysis of the Role of PUB1 in Nodulation using Overexpression and Knockdown Strategies.

Graphs represent the nodulation efficiency ratios: average number of nodules in the assay versus average number of nodules in the control. Each ratio is calculated using data from two independent experiments shown in Tables 1 and 2. The dashed line represents no difference of the assay plants to the vector control plants. *, Data showing statistical differences (P values below 0.01) in replicate experiments (see Tables 1 and 2).

(A) Overexpression of 3HA-PUB1 induces a significant delay in nodulation. M. truncatula wild-type plants, overexpressing 3HA-PUB1 or GFP (control) under the control of Pro35S, were inoculated with wild-type S. meliloti, and nodules were scored at 7 and 14 DAI (dpi) in growth pouches.

(B) PUB1-RNAi plants show increased nodulation efficiency of M. truncatula wild-type plants with S. meliloti strains altered in NF structure and of M. truncatula lyk3-4 plants with the S. meliloti wild-type strain. M. truncatula wild-type or lyk3-4 plants, transformed with the PUB1-RNAi construct or vector control, were inoculated with wild-type, nodL, or nodFnodL S. meliloti strains. Nodules were scored at 21 DAI in pots.