Table 1.
Segregation Analysis of sdg2 Mutant Alleles in Progeny Derived from Self-Pollination or Crosses
| Parent | Progenyab | Observed Ratioc | Expected Ratio | |
| Self-Pollination | S | R | S:R | S:R |
| SDG2-1+/− selfing | 657 | 1099 | 1:1.67*** | 1:3 |
| SDG2-2+/− selfing | 2093 | 4756 | 1:2.27*** | 1:3 |
| SDG2-3+/− selfing | 1337 | 2738 | 1:2.05*** | 1:3 |
| Crosses | SS | Ss | SS:Ss | SS:Ss |
| Col (♀) × SDG2-1+/− (♂) | 74 | 43 | 1:0.58** | 1:1 |
| Col (♀) × SDG2-3+/− (♂) | 62 | 34 | 1:0.55** | 1:1 |
| SDG2-1+/− (♀) × Col (♂) | 158 | 130 | 1:0.82− | 1:1 |
| SDG2-3+/− (♀) × Col (♂) | 161 | 127 | 1:0.79* | 1:1 |
Alleles of sdg2-1, sdg2-2, and sdg2-3 are associated with phosphinothricin, sulfadiazine, and kanamycin resistance marker genes. Numbers of sensitive (S) and resistant (R) plants grown on selective media are used to investigate transmission of mutant alleles.
Wild-type SDG2 (S) and mutant sdg2 (s) alleles were determined by PCR analysis. Numbers of plants with Col (SS) and SDG2-1+/− or SDG2-3+/− (Ss) genotypes are shown.
The ratios obtained from experimental data are lower than those expected from normal segregation, indicating reduced transmission efficiency of mutant alleles. Statistical significance: ***P < 0.001; **P < 0.01; *P < 0.05; −P > 0.05.