Figure 4.

SNP2 (D₁₉₁) Cosegregates with Colored Roots in a Cassava Pedigree.

Cultivars MBRA1A (yellow-rooted) and MMAL66 (white-rooted) were crossed using MMAL66 as the male parent. Siblings of this cross were self-pollinated, and the resulting offspring grown in the field. Randomly selected white and colored roots were harvested after 9 months of growth and analyzed for carotenoid content and composition. Leaf DNA was used for SNP2 identification through sequencing of the respective PCR-amplified PSY region. The presence of Asp at position 191, replacing the conserved Ala, was decisive for carotenoid accumulation (see Supplemental Table 2 online).