Figure 2.

Expression Levels of Clade 2 ERF Genes Are Affected in nic Mutant Roots.

Transcript levels of clade 2 ERFs, ERF10 (clade 1), and PMT in the tobacco roots of different NIC genotypes were measured by qRT-PCR and are shown relative to the wild-type levels. ERF115/29/221/104/16/17, ERF179/130/168, and ERF163/210 were collectively amplified by the PCR primers used (see Supplemental Figure 3 online for breakdown of the amplified ERF proportions). Error bars represent sd of three replicates. Significant differences among the genotypes at P < 0.05 were determined by one-way analysis of variance (ANOVA) followed by the Tukey-Kramer test and are indicated by different letters. ND, not detected.