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. 2010 Oct 19;22(10):3390–3409. doi: 10.1105/tpc.110.078543

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© 2010 American Society of Plant Biologists

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Figure 3. — Seven ERF Genes Originating from N. tomentosiformis Are Absent in nic2 Mutants.

(A) Genomic PCR analysis of clade 2 ERF genes. Genomic DNA templates from tobacco (N. tabacum; cv Burley 21 of the wild type, nic2, nic1, and nic1 nic2 and cv NC 95 of the wild type and nic1 nic2), N. sylvestris (N. syl), and N. tomentosiformis (N. tom) were amplified with the primers specific to the indicated ERF genes. The tobacco α-tubulin gene was analyzed as a control.

(B) Genomic DNA gel bolt analysis of clade 2 ERF genes. Genomic DNA samples from tobacco (the wild type and nic2), N. sylvestris (N. syl), and N. tomentosiformis (N. tom) were digested by HindIII, blotted on a membrane, and probed with cDNA fragments from ERF189, ERF221, or ERF179, which showed high, but not exclusive, specificity to the target genes (see Supplemental Figure 4A online). DNA fragments missing in nic2 are marked by arrowheads. The numbers on the left sides of blots show the sizes of marker fragments in kilobases. The hybridization data for clade 2-3 ERFs are available in Supplemental Figure 4B online.