Figure 5.

Genotypes and Phenotypes in Two F2 Progeny (F2#3 and F2#12) Derived from Dmt102/dmt102::Mu Plants Carrying the RNAi Transgene from D103-4.

RT-PCR was used to determine expression of Dmt103 in F2 plants; four technical replicates were performed for each F2 plants. Genotypes at the dmt102 locus were determined by PCR: wild type (+/+), heterozygous (+/−), or homozygous recessive (−/−). Herbicide resistance tests indicated resistant (R) and susceptible (S) plants. Ovule, ES, and pollen defects were scored as positive (Y) or negative (N), depending on whether some of the defects observed in single dmt- mutants were observed. nd, not determined.