Table 1.
Substrate Preferences and Relative Activities of AAGTs for Various Donor Molecules
| nkat/mg Protein (Relative Activities [%]) | |||
| Substrates | Native Dc AA5GT | Recombinant Dc AA5GT | Recombinant Dg AA7GT |
| Phenol glucosides | |||
| 1-O-β-d-Vanillyl-glucose | 0.34 ± 0.04 (100) | 0.76 ± 0.09 (100) | 1.02 ± 0.08 (100) |
| 1-O-α-d-Vanillyl-glucose | ND | ND | ND |
| 1-O-β-d-Isovanillyl-glucose | 0.11 ± 0.03 (31) | 0.17 ± 0.05 (22) | 0.27 ± 0.06 (26) |
| 1-O-β-d-Galloyl-glucose | ND | ND | ND |
| 1-O-β-d-p-hydroxybenzoyl-glucose | 0.08 ± 0.01 (22) | 0.07 ± 0.01 (9) | 2.2 ± 0.1 (146) |
| 1-O-β-d-Sinapoyl-glucose | 2.8 ± 0.2 (827) | 12.6 ± 0.2 (1663) | ND |
| 1-O-β-d-Feruloyl-glucose | 4.4 ± 0.1 (1284) | 14 ± 1 (1834) | 0.04 ± 0.01 (4) |
| 1-O-β-d-Caffeoyl-glucose | 1.0 ± 0.1 (295) | 1.90 ± 0.06 (250) | 0.02 ± 0.01 (2) |
| 1-O-β-d-4-Coumaroyl-glucose | 1.5 ± 0.3 (442) | 3.69 ± 0.16 (485) | 0.08 ± 0.02 (8) |
| 1-O-β-d-Vanillyl-galactose | ND | ND | ND |
| p-Nitropheny-glucoside | ND | ND | ND |
| Arbutin | ND | ND | ND |
| Salicin | ND | ND | ND |
| Populin | ND | ND | ND |
| Eleutheroside B | ND | ND | ND |
| Phloridzin | ND | ND | ND |
| Glucovanillin | ND | ND | ND |
| Coumarin glucoside | |||
| Esculin | ND | ND | ND |
| Flavonoid glucosides | |||
| Kaempferol 3-glucoside | ND | ND | ND |
| Kaempferol 7-neohesperidoside | ND | ND | ND |
| Apigenin 7-glucoside | ND | ND | ND |
| Naringenin 7-glucoside | ND | ND | ND |
| Daidzin | ND | ND | ND |
| Indole glucoside | |||
| Indican | ND | ND | ND |
Donor preferences of AAGT proteins using Cy3G as the acceptor. Relative activity is shown in comparison to 1-O-β-d-vanillyl-glucose, which is arbitrarily set as 100%. All values are the mean ± sd at least three independent determinations. ND, not detectable.