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. 2010 Dec 15;137(24):4159–4169. doi: 10.1242/dev.056630

Fig. 2.

Fig. 2.

Significant effect of Cdx2 reduction on gene expression at various developmental stages but no blockage of expression of the differentiated TE marker Krt8. (A) Relative gene expression levels as a percentage of peak expression of Cdx2, Eomes, Fgfr2 and Oct4 in mouse preimplantation embryos after siControl and siCdx2 injection into zygotes. The downstream transcription factor Eomes was reduced to a basal level. Fgfr2 reduction is shown at the early and expanded blastocyst stages. Oct4 expression was increased ∼2-fold in both the early and expanded blastocyst stages (P<0.0001). Error bars indicate standard deviation. (B) Ectopic expression of Nanog in the TE of Cdx2-deficient blastocysts after microinjection of siCdx2 at the zygote stage. The embryonic stem cell marker Nanog was ectopically expressed in TE, as shown in this z-stack confocal image of an immunostained siCdx2-treated blastocyst. (C) Sectional confocal image showing the presence of Krt8 (as detected by the Troma-1 antibody) in an interrupted pattern in Cdx2 knockdown blastocysts.