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. 2010 Dec 15;137(24):4159–4169. doi: 10.1242/dev.056630

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Fig. 6. — Effective knockdown of Tead4 in preimplantation mouse embryos by microinjection of siRNA into zygotes reproduces the Tead4^–/– embryo phenotype. (A) Tead4 knockdown efficiency (%) determined by qRT-PCR in a single E4.5 mouse blastocyst in comparison to the scrambled siRNA control. Error bars indicate standard deviation. (B) Failure of blastulation 72 hours (E3.5) after microinjection of siTead4A and siTead4B into mouse zygotes. In this particular experiment, 25 zygotes per group were successfully injected. Note that the phenotype is consistent and highly reproducible within the siTead4A and siTead4B groups (except for one embryo in each group that was blocked at the 2-cell stage), but was variable in groups with lower Tead4 knockdown efficiency. (C) Tead4 knockdown reduced the expression of genes crucial in TE lineage differentiation but did not reduce the expression of pluripotency-related genes. Shown are qRT-PCR results from pools of six embryos, with biological and technical triplicates.