Fig. 5.

dFMRP and CAPR associate with CycB and frs mRNAs in wild-type embryos and specifically alter CYCB and FRS protein expression at the MBT. (A) An immunoblot of ten Drosophila embryos per lane of the indicated genotypes (top) was probed for the indicated proteins (left), which included Lamin (LAM) as a loading control. Hand-sorted embryos were of the indicated stages: NC13 interphase (I13) and mitosis (M13), or NC14 early, middle and late interphase (I14). Phospho-isoforms of CDC2 (Edgar et al., 1994) are labeled (1-4). Red boxes highlight differences in protein expression between genotypes. (B) mRNA specifically immunoprecipitating from NC13 to early NC14 embryo extracts with CAPR or dFMRP as determined by quantitative PCR. Levels are presented as ratios (wild type/mutant) of RpL32 (control), CycB and frs mRNAs in immunoprecipitates. Error bars indicate s.d. Significance was determined for normalized mRNA values using a two-tailed Student's t-test. ^*, P=0.022 for CycB and P=0.020 for frs; ^**, P=0.003 for CycB and P=0.00005 for frs.