Figure 5. SNDX-275 enhances melphalan-induced mitotic catastrophe in MM cells.
MM1.S cells were cultured with RPMI1640 (0.5% FBS) in the absence or presence of SNDX-275 (0.5 µM), melphalan (10 µM) alone or combinations of SNDX-275 and melphalan for 24 hrs. Cells were harvested and subjected to cytospin onto cell slides. A, Slides were stained with HE, examined, and pictures were taken under a photomicroscope. B, Pathologist was blinded on each slide set regarding the treatment groups. Cells that showed atypical mitotic figures, multi-nucleation, atypical chromosome clusters, and apoptosis were counted against normal cells, and reported in percentage.