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Verification of pRNA structure and function in (A−B) dimer formation and (C) procapsid binding after modification. (A) Native gel electrophoresis of modified pRNAs, compared with unmodified pRNAs. (B) Fluorescent gel images of modified pRNAs, compared with unmodified pRNAs. (C) Comparison of procapsid binding activities by [3H] counting for the modified pRNAs with unmodified pRNAs. The number of Nt indicates the position of the nucleotides for fluorescent labels.
