TABLE 1.
Proteins of interest that interact with CsdE-(His)6a
| Number of Unique Peptidesb Gel Section |
||||||||
|---|---|---|---|---|---|---|---|---|
| Function | MW | Fe-S (Ref) | 1 | 2 | 3 | 4 | 5 | 6 |
| Iron-sulfur cluster assembly | ||||||||
| ErpA | 12 kDa | 3 | ||||||
| SufE | 16 kDa | 4 | 4 | 3 | 2 | 2 | ||
| CsdA | 43 kDa | 4 | 18 | 12 | 30 | 20 | 8 | |
| CsdE | 16 kDa | 65 | 59 | 43 | 25 | 32 | 11 | |
| IscS | 45 kDa | 4 | ||||||
| Porphyrin metabolism | ||||||||
| HemL | 45 kDa | 6 | ||||||
| Cellular redox homeostasis | ||||||||
| GrxC | 9 kDa | 2 | ||||||
| GrxD | 13 kDa | Yes (64,65) | 4 | |||||
| TrxA | 12 kDa | 3 | ||||||
| AhpC | 21 kDa | 2 | 16 | |||||
| Oxidative phosphorylation | ||||||||
| NuoI | 21 kDa | Yes (52) | 2 | |||||
| NuoF | 49 kDa | Yes (51) | 2 | |||||
| Gluconeogenesis | ||||||||
| SdaB | 49 kDa | Yes (53) | 6 | |||||
| Amino acid metabolism | ||||||||
| TnaA | 53 kDa | 2 | 4 | 5 | 27 | 3 | ||
| Uncharacterized | ||||||||
| YchN | 13 kDa | 2 | ||||||
| CsdL | 29 kDa | 2 | ||||||
a
Protein identification probability is 100% for all proteins except for CsdL, gel section 3, where it is 98%
b
All peptides identified with ≥95% confidence