Table 2.

Nitric oxide release studies for NONO-NSAIDs 7–9.

Compound	Nitric oxide releaseda
	PBS (pH 7.4)b	t (h)c	PLEd	t (min)e
7	1.0f	40.0	2.0f	6.5
8	1.1f	45.3	2.0f	0.6
9	0.9g	48.0	2.0g	1.4
12	1.0f	30.7	2.0f	2.4

^aMoles of •NO/mol of test compound.

^bIncubated in phosphate buffer solution pH 7.4 at 37 °C.

^cTime required to detect about 50 % of the theoretical total amount of •NO/mol of test compound. Calculated graphically from the corresponding curves plotting time vs. mol •NO/min.

^dMoles of •NO released in the presence of porcine liver esterase (10 μL of a suspension in 3.2 M (NH₄)₂SO₄, Sigma).

^eTime required to detect 100 % of the theoretical total amount of •NO/mol of test compound. Calculated graphically from the corresponding curves plotting time vs mol •NO/min.

^f10 μL of a 46 mM solution in 3 mL of phosphate buffer pH 7.4.

^g10 μL of a 77 mM solution in 3 mL of phosphate buffer pH 7.4.