Fig. 1.

Schematic diagram of mouse JP2 primary sequence, and co-immunoprecipitation of TRPC3 and GST-JP2. a Eight MORN motifs and a transmembrane (TM) domain exist. Amino acids are numbered. ‘+’ or ‘−’ indicates positively or negatively charged residue. b Solubilized rabbit triad sample containing intact TRPC3 was incubated with E. coli lysate expressing GST-JP2 and then subjected to co-immunoprecipitation assay with anti-TRPC3 antibody followed by immunoblot assay with anti-TRPC3, anti-GST, or anti-JP2 antibody. GST-JP2 was successfully co-immunoprecipitated with TRPC3. To show the specificity of anti-TRPC3 antibody for the recognition of TRPC3, “Input” without antibody served as a negative control for co-immunoprecipitation assay in Figs. 1–5. IP, immunoprecipitation; IB, immunoblot