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. Author manuscript; available in PMC: 2010 Nov 23.
Published in final edited form as: Mol Cell Biochem. 2009 Mar 10;328(1-2):25–32. doi: 10.1007/s11010-009-0070-0

Fig. 2.

Fig. 2

Co-immunoprecipitation of TRPC3 and GST-JP2 fragments. a Successful expression of GST-JP2 fragments (F1 and F2) was confirmed by immunoblot assay with anti-GST antibody. b Solubilized rabbit triad sample containing intact TRPC3 was incubated with E. coli lysate expressing F1 or F2, and then subjected to co-immunoprecipitation assay with anti-TRPC3 antibody followed by immunoblot assay with anti-TRPC3 or anti-GST antibody. F1 was strongly co-immunoprecipitated with TRPC3 but not F2. IP, immunoprecipitation; IB, immunoblot