Figure 2.
Defective DNA methylation of Dicer1-null cells is corrected by Dnmt overexpression. (a) Quantification of Dnmt mRNA levels. (b) Representative western blots. Dnmt1- and Dnmt3a,3b-deficient cells were used as negative controls and Dnmt3a,3b-deficient cells were reconstituted with the Dnmt3a enzyme (Dnmt3a,3b−/−* Dnmt3a) as a control for Dnmt3a mobility in the gel. (c) Quantification of western blot results shown in part b. (d) Dnmt mRNA levels before or after expression of the indicated enzymes. (e) Fraction of methylated B1 SINE repeat element in two Dicer1-null cultures (27H10 and 27G5) before and after Dnmt overexpression. (f) Percentage of CpG methylation at the indicated subtelomeres before and after overexpression of the indicated Dnmts. Bisulfite sequencing of 2–16 individual clones was performed.