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. Author manuscript; available in PMC: 2010 Nov 23.
Published in final edited form as: Nat Cell Biol. 2008 Nov 30;11(1):56–64. doi: 10.1038/ncb1812

Figure 5.

Figure 5

Repair patch formation by MG53 restores cell integrity following acute injury. (a) GFP–MG53 expressed in mg53−/− myotubes translocates to cell injury site after UV-bleaching (left), whereas GFP–C242A remains immobile after photobleaching (right). (b) mg53−/− myotubes transfected with GFP–MG53 (left) or GFP–C242A (right) were assayed for FM4-64 dye entry after UV-laser damage. Arrows indicate the site of laser damage. (c) GFP–MG53 expression (blue) can prevent FM4-64 dye entry, whereas GFP–C242A cannot (red). Data represent mean ± s.e.m. (n = 9). (d) FDB muscle fibres from WT mice were transfected by in vivo electroporation to allow for transient expression of GFP–MG53 (left) and GFP–C242A (right) (see also Supplementary Information, Fig. S5). (e) FDB muscle fibres transfected with GFP–C242A show excessive FM4-64 dye entry following UV-laser wounding (right), compared with those transfected with GFP–MG53 (left; n = 15). (f) Summary data for panel e. Data represent mean ± s.e.m., n = 8.