. Author manuscript; available in PMC: 2010 Nov 23.

Published in final edited form as: Nat Protoc. 2009 May 28;4(6):960–974. doi: 10.1038/nprot.2009.68

TABLE 1.

Genomic DNA oligonucleotide sequences.

PCR primers (50 µM each)
PCR primer 1.1	5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT–3′
PCR primer 2.1	5′-CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-3′
Blocking oligonucleotide (BO) mix (200 µM each)
BO 1 (PCR primer 1.1)	5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′
BO 2 (PCR primer 2.1)	5′-CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-3′
BO 3 (reverse complement 1.1)	5′-AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT 3′
BO 4 (reverse complement 2.1)	5′-AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG-3′