| Reagent | Volume (µl) per sample | Final concentration |
|---|---|---|
| Phusion DNA polymerase (2×) | 25.0 | 1× |
| PCR primer 1.1 (50 µM) | 1.0 | 1 µM |
| PCR primer 2.1 (50 µM) | 1.0 | 1 µM |
| Water | 18.0 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.