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. Author manuscript; available in PMC: 2010 Nov 23.
Published in final edited form as: Nat Protoc. 2009 May 28;4(6):960–974. doi: 10.1038/nprot.2009.68
Reagent Volume (µl) per sample Final concentration
SYBR Green PCR master mix (2×) 10.0 1×
Forward primer (10 µM) 0.5 0.25 µM
Reverse primer (10 µM) 0.5 0.25 µM
Template (20 ng µl−1) 1.0 (1 ng µl−1)
Water 8.0