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. Author manuscript; available in PMC: 2010 Nov 23.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2006 Nov 16;27(2):422–429. doi: 10.1161/01.ATV.0000252826.96134.21

Figure 1.

Figure 1

The requirement for Syk in spreading is by-passed by thrombin. Glass coverslips were coated with VWF plus botrocetin (10 µg/mL), collagen (100 µg/mL), CRP (100 µg/mL), fibrinogen (FGN; 200 µg/mL), or fibronectin (FN; 100 µg/mL). Nonimmobilized protein was removed and coverslips were blocked with BSA (5 mg/mL). Platelets from wild-type (WT) or Syk−/− mice were incubated on the coverslips for 45 minutes in the absence (A) or presence (B) of thrombin (1.0U/mL). Nonadherent platelets were removed and adherent platelets fixed then imaged using DIC microscopy. Scale bar=10 µm. Representative images are shown from 2 to 3 experiments performed in duplicate, with 5 fields taken per image.