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. Author manuscript; available in PMC: 2010 Nov 23.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2006 Nov 16;27(2):422–429. doi: 10.1161/01.ATV.0000252826.96134.21

Figure 5.

Figure 5

CRP potentiates thrombin-induced integrin αIIbβ3 activation through GPVI. Platelet suspensions from wild-type mice were treated with PP2 (10 µmol/L, black), and compared with murine suspensions of (A) GPVI−/− or (B) FcRγ chain−/− platelets (gray). Platelets were allowed to rest in the presence of vehicle (Resting), or were stimulated with thrombin (0.03 or 0.05U/mL), CRP (2 µg/mL), or CRP/thrombin (2 µg/mL and 0.03 or 0.05U/mL, respectively). Some samples were treated with EGTA/Mg2+ and stimulated with thrombin (0.04 U/mL) to block integrin activation. Suspensions were incubated with OG-FGN and samples read in FL1. Results show integrin αIIbβ3 activation, measured in arbitrary fluorescence units. A, Data are from single data points from one experiment, representative of 2 to 3 experiments (WT=3, GPVI−/−=2). B, Data are pooled from triplicate samples from 3 individual experiments (mean ± SEM).