Figure 4.
Time course of 6-HO-PdG, 8-HO-PdG, 8-dG-AC-dG (measured as the reduced, 8-dG-(CH2)3-dG cross-link), HNE-dG and 8-dG-HNE-dG cross-link formation in CT DNA. Panel A: 1 mol AC reacted per mol base in CT DNA over a time period of 0, 1, 2, 3, 6 and 10 days; Panel B: Levels of 6-HO-PdG, 8-HO-PdG and 8-dG-AC-dG (measured as the reduced, 8-dG-(CH2)3-dG cross-link) formed in CT DNA treated with 0.1, 0.5, 1 or 5 mol AC per mol base in CT DNA for 10 days; and Panel C: Time course of HNE-dG and 8-dG-HNE-dG cross-link formation in CT DNA treated with 2 mol HNE per mol base in CT DNA over a time period up to 21 days.The upper panels depict the monomeric DNA adducts and lower panels depict the cross-linked DNA adducts.