Figure 6.
Reconstructed ion chromatograms of the LC-ESI/MS/MSn traces of CT-DNA treated with HNE. A reconstructed ion chromatogram of the LC-ESI/MS/MS3 trace for HNE-dG and the reconstructed ion chromatogram of the LC-ESI/MS/MS4 trace for the 8-dG-HNE-dG cross-link. Both [2H11]-HNE-dG and [2H11]-8-dG-HNE-dG cross-link were added to CT DNA at a level of 1 adduct per 107 bases. Panel A depicts untreated CT DNA and Panel B depicts CT DNA treated with 2 mol of HNE per mol base in CT DNA.