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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Chem Res Toxicol. 2010 Oct 22;23(11):1701–1713. doi: 10.1021/tx100179g

Table 3.

AC-DNA Adduct Formation in Untreated CT DNA versus CT DNA that was “Purified” by Treatment with S1 Nuclease, followed by ProCipitate™.

Sample Days 8-HO-PdGred× 10−5 dG-(CH2)3-dG × 10−7 PdG × 10−6
CT DNA 2 3.9 ± 0.2* 3.6 ± 0.6** 14.8 ± 0.9**
Purified- CT
DNAa 		2 3.5 ± 0.1 7.6 ± 0.9 2.6 ±0.2
CT DNA 10 11.3 ± 1.3* 11.4 ± 1.3** 33.7 ± 4.5**
Purified-CT
DNAa 		10 9.7 ± 1.6 21.9 ± 3.9 7.3 ± 0.9

N = 6 independent DNA binding experiments with AC

Unpaired t test (2-tailed)

*

P < 0.014: AC adduct formation from CT DNA versus Purified CT DNA

**

P < 0.001: AC adduct formation from CT DNA versus Purified DNA