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. Author manuscript; available in PMC: 2010 Nov 23.
Published in final edited form as: Cell Death Differ. 2009 Apr 17;16(8):1167–1179. doi: 10.1038/cdd.2009.37

Figure 5. Arachidonic acid or 12-lipoxygenase induced cell death was attenuated by 12-lipoxygenase inhibitors.

Figure 5

Cultured HT4 cells were treated with 50 μM BSO for 12h (A to L), and either 0.15 attomole arachidonic acid (A, B, and C) or 5 ×10−8 units 12-Lox (D, E, and F) were injected (marked by arrow) into cytoplasm (A and D, without treatment; B and E, 2.5 μM BL-15 added to culture medium prior to microinjection; C and F, 1 μM TCT added to culture medium prior to microinjection). After 24h incubation, cells injected with arachidonic acid (G) lost cell viability, while BL-15 (H) or TCT (I) treated cells were protected, and cells injected with 12-Lox (J) lost cell viability, while BL-15 (K) and TCT (L) treated cells were protected (M). attomoles injected [micromolar]i, n=3, Bar, 20 μm. *, lower than control, †, higher than cells without treatment of 12 Lox inhibitors. Results are mean ± S.D. *, p < 0.05.