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. Author manuscript; available in PMC: 2010 Nov 23.
Published in final edited form as: Cell Death Differ. 2009 Apr 17;16(8):1167–1179. doi: 10.1038/cdd.2009.37

Figure 6. Bischloroethylnitrosourea induced cell death was attenuated by 12-lipoxygenase inhibitors.

Figure 6

HT4 cells were treated with ethanol (A), 50 μM 1,3-Bis (2-chloroethyl)-1-nitrosourea; BCNU (B), 50 μM BCNU and 2.5 μM BL-15 (C), or 50 μM BCNU and 1 μM TCT (D). After 12h of BCNU treatment, live cells were visualized using calcein-AM (A–D); cell viability was also assayed using a calcein AM based cell viability kit (E). Cells treated with BL-15 or TCT were more resistant to BCNU-induced loss of cell viability. BCNU treatment increased cellular GSSG over time (F). G, MK571 (20μM) was added to HT4 cell culture medium for 6h prior to 50 μM BCNU treatment. After 12h of adding BCNU into cell culture medium, loss of cell viability was assessed by measuring leakage of lactate dehydrogenase (LDH). MK571 treated HT4 cells were more vulnerable to BCNU-induced loss of cell viability. n=3, Bar, 100 μm. *, lower than control, †, higher than BCNU-challenged HT4 cells, §, higher than control. Results are mean ± S.D. *, p < 0.05.