Figure 7. In vitro differentiation using embryoid bodies from hiPSCs generated and maintained in defined culture conditions.

Immunohistochemistry of MAP2 (A), TUJ (B), FLK1 (C), VIMENTIN (D), and PDX1 (E) in the differentiated hiPSC line, UTA-SF-2-2, grown under hESF9a-based conditions. Differentiation was performed using embryoid body formation. The tissues in embryoid bodies were fixed and reacted with antibodies. Binding of these antibodies was visualized with AlexaFluor 488-conjugated secondary antibodies (green). Nuclei were stained with DAPI (blue). Scale bars are 50 µm.