Skip to main content
. 2010 Nov 23;5(11):e14102. doi: 10.1371/journal.pone.0014102

Figure 2. Ash2L/RbBP5 has intrinsic methyltransferase activity.

Figure 2

(A) In vitro HMT assay using ∼5 µM Ash2L/RbBP5 heterodimer as enzymes. Top, fluorogram of methylated H3 after three weeks of exposure. Bottom, Coomassie stained gels for H3 substrate. (B) In vitro HMT assay using 60 µM unmodified, mono-, di- and tri-methylated H3 K4 peptides as substrates. The proteins (∼5 µM final concentration) in each reaction were indicated on bottom. In vitro HAT assay using p300 was included as a quality and loading control for H3 peptides. The exposure times for the top and bottom panels were different and were indicated on bottom. (C) Left, in vitro HMT assay for Ash2L, Ash2L/RbBP5 as well as Ash2LSPRY/RbBP51-410. Right, in vitro HMT assay for Ash2LSPRY and various RbBP5 peptides as indicated on top. Coomassie stained gels for H3 and proteins used in the assays were included on bottom.