Skip to main content
. 2010 Nov 23;5(11):e13804. doi: 10.1371/journal.pone.0013804

Figure 5. Cytokine expression profile of S.TmSipA- and S.TmSopE-infected Il17ra+/−, Il17ra−/− and C57BL/6 mice.

Figure 5

Salmonella-infected animals (n = 3) from experiment shown in Fig. 2 were used for analysis of gene expression signature. Cecum samples from Il17ra−/−, Il17ra+/−, and C57BL/6 mice 12 h p.i. with S.TmSipA or S.TmSopE, were taken for RNA isolation and RT-PCR (A–B). 45 genes related to inflammation and defense, especially in IL-17-mediated responses were used. The analysis was performed using RealTime StatMiner Software; mRNA levels were normalized to 3 house-keeping genes: Actb, Gapdh and 18S (ΔCt). Relative quantification was determined by the ΔΔCt method after normalization to streptomycin-treated C57BL/6 animals. Statistical analysis was done using the Limma test with Bonferroni correction (A–B). All results presented in panels A and B did not differ significantly between Il17ra−/− and Il17ra+/− mice (p≥0.05) with the exception of Gem and Nos2 in panel A (*: p<0.05).