Figure 5.

Silver stained 2D-PAGE profile of crude canine follicular fluid. 100 μg of proteins samples were applied to a non-linear pI 3-10 IPG strip in the first dimension and separated on a 10% SDS-PAGE gel in the second dimension (Molecular weight 200-10 kDa range). The positions of the high-abundant proteins albumin (ALB) and immunoglobulin heavy chain (HV01 and HV02), are indicated by dark full and dotted rectangles. Additional albumin fragments are indicated by dark circles (see Additional file 1, Table S1). All other identified proteins are named on the figure and their characteristics are shown in Table 1. NI: non-identified proteins