Table 1.
Identification by mass spectrometry of canine follicular fluid proteins separated by 2D-PAGE
| Gene | AC1 N° | Protein name | Exp2 | Theo2 | Score3 | P N°2 | SC (%)4 |
|---|---|---|---|---|---|---|---|
| pI2 /Mr2 | |||||||
| A2AP | P28800 | Alpha-2-antiplasmin | 6,08/57 | 5.36/54 | 10.17 | 1 | 2.2 |
| ACTG | Q9UVW9 | Actin-gamma | 5,49/42 | 5.36/41 | 20.22 | 2 | 4.8 |
| AHSG | P12763 | Alpha-2-HS-glycoprotein | 4,66/57 | 5.16/38 | 10.14 | 1 | 3.6 |
| APOA1 | P02648 | Apolipoprotein A-I | 5,05/48 | 5.06/30 | 10.16 | 1 | 3.4 |
| APOA1 | P02648 | Apolipoprotein A-I | 5,07/24 | 5.06/30 | 296.41 | 30 | 72.2 |
| APOA1 | P02648 | Apolipoprotein A-I | 6,20/21 | 5.06/30 | 50.23 | 5 | 24.1 |
| APOA1 | P02648 | Apolipoprotein A-I | 5,07/62 | 5.06/30 | 40.24 | 4 | 14.3 |
| APOA4 | Q32PJ2 | Apolipoprotein A-IV | 5,44/38 | 5.17/42 | 50.21 | 5 | 9.7 |
| AT3 | P41361 | Antithrombin-III | 5,14/55 | 7.08/52 | 80.20 | 8 | 8 |
| AZGP1 | Q3ZCH5 | Zinc-alpha-2-glycoprotein | 4,8/33 | 4.98/33 | 30.14 | 3 | 10.4 |
| C4 | P01030 | Complement C4 | 7,48/31 | 6.13/101 | 34.19 | 4 | 4 |
| CFB | P81187 | Complement factor B | 7,04/59 | 7.07/85 | 20.21 | 2 | 3.8 |
| CFB | P81187 | Complement factor B | 6,38/102 | 7.5/85 | 20.26 | 2 | 3.8 |
| CLU | P25473 | Clusterin | 5,05/33 | 5.56/51 | 10.16 | 1 | 3.6 |
| CLU | P25473 | Clusterin | 5,3/31 | 5.56/51 | 20.16 | 2 | 6.1 |
| FGG | P02679 | Fibrinogen gamma chain | 5,8/48 | 5.27/51 | 56.25 | 6 | 14.3 |
| GC | Q3MHN5 | Vitamin D-binding protein | 5,17/54 | 5.24/53 | 70.29 | 7 | 9.5 |
| GSN | Q3SX14 | Gelsolin | 6,01/88 | 5.45/80 | 20.24 | 2 | 5.5 |
| HP | P19006 | Haptoglobin | 5,31/33 | 5.68/36 | 60.21 | 6 | 15.8 |
| HP | P19006 | Haptoglobin | 5,47/33 | 5.68/36 | 50.30 | 5 | 21 |
| HPR | Q28801 | Haptoglobin-related protein | 5,75/33 | 7.61/38 | 10.15 | 1 | 5.8 |
| ICA | Q29545 | Inhibitor of carbonic anhydrase | 6,29/77 | 5.85/77 | 50.20 | 5 | 3.6 |
| PON1 | P27169 | Paraoxonase/arylesterase 1 | 5,13/42 | 4.96/39 | 20.27 | 2 | 7.9 |
| RBP4 | P18902 | Retinol-binding protein 4 | 5,45/20 | 5.33/21 | 40.21 | 4 | 12 |
| TF | Q29443 | Serotransferrin | 7,05/72 | 6.69/77 | 42.25 | 5 | 7.7 |
The experimental position of identified spots is shown in Figure 5.
1 AC is an accession number from the UniProt database
2 Exp: Experimental; Theo: Theoretical; pI: Isoelectric point; Mr: Molecular weight; P N°: Peptid number; SC: Sequence coverage
3 Score is −10*Log (P), where P is the probability that the observed match is a random event
4 Sequence coverage (%) is based on number of peptide masses matched in UniProt databases