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. 2010 Nov 23;5(11):e15481. doi: 10.1371/journal.pone.0015481

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Comparison of the prediction performance of Pfam-based prediction (PFANTOM), all predictors by SUBA database, and WoLF PSORT. NC, nucleus; MT, mitochondrion; V, vacuole; PX, peroxisome; ER, endoplasmic reticulum; GO, Golgi apparatus; CT, cytosol; PM, plasma membrane; PL, plastid; EX, extracellular. (B-T) Subcellular localization of transiently expressed YFP-fusion proteins in N. benthamiana. (B) bHLH (AT5G48560); (C) SND1 (AT1G32770); (D) Golgi marker (STtmd-GFP); (E) ER marker (GFP-HDEL); (F) Plasma membrane marker (pm-rk); (G) PGSIP1 (AT3G18660); (H) PGSIP3 (AT4G33330); (I) TBL3 (AT5G01360, belonging to DUF231); (J) Unknown protein (AT2G38320, belonging to DUF231); (K) CTL2 (AT3G16920); (L) FLA11 (AT5G03170); (M) FLA12 (AT5G60490); (N) COBL4 (AT5G15630, IRX6); (O) LRR protein (AT1G67510); (P) NCRK (AT2G28250); (Q) RIC2 (AT1G27380); (R) RIC4 (AT5G16490); (S) ROP7 (AT5G45970); (T) ROPGEF4 (AT2G45890). A summary of the localization experiments is shown in Table 2. Scale bar = 20 µm.