Figure 5. Gating strategy in myeloid/monocytic lineage tubes.

Single-cell suspensions from healthy donors were stained with a combination of 14 antibodies and viability dye as described in the material and methods and table 1. Monocytes were identified based on their forward and side scatter properties. Subsequently, dead cells were excluded through the use of a viability dye. CD45 and CD14 were used to identify monocytes (CD45⁺CD14⁺) among the previously selected living monocytic gate.