. Author manuscript; available in PMC: 2012 Jan 5.

Published in final edited form as: J Immunol Methods. 2010 Jun 25;363(2):245–261. doi: 10.1016/j.jim.2010.06.010

TABLE 1.

Markers and fluorochromes used in preliminary panel

	T lineage			B lineage			NK lineage		Dendritic lineage	Mono lineage

Fluorochrom	T_reg	T_h17	T_h1/h2	B_general	B_naive/memory	B_{intracellular}	NK₁	NK₂	DC₁	M₁
V450	CD4	CD4	CD4	Lambda	CD80	CD80	CD56	CD56	CD123	HLA-DR
Aquablue	Viability	Viability	Viability	Viability	Viability	Viability	Viability	Viability	Viability	Viability
Qdot 605	CD8	CD8	CD8	CD10	CD27	CD27	CD8	CD8	CD86	CD163
Qdot 655	CD27	CD27	CD27	CD19	CD19	CD19	CD30	NKp46	CD4	CD14
Qdot 800	CD45	CD45	CD45	CD45	CD45	CD45	CD45	CD45	CD45	CD45
FITC	CD39	IL-23R	CD69	CD40	IgA	IgA	CD57	CD57	IFNa	CD77
PcP-Cy5.5	CD38	CD196	IL-4	CD138	CD86	CD86	CD161	IFNg	TNFa	CD192
PE	FOxp3	IL-22	Perforin	Kappa	CD21	IgE	CD337	Perforin	CD40	CD64
PE-TR	CD45-RA	CD45-RA	CD45-RA	CD38	CD38	CD38	CD94	CD25	HLA-DR	CD16
PE-Cy5	CD103	CD161	CD40L	CD103	IgG	IgG	CD16	CD16	CD83	CD15
PE-Cy5.5 /PE-A700	HLA-DR	TNFa	TNFa	CD20	CD20	CD20	CD244	CD69	CD11c	CD4
PE-Cy7	CD25	IL-17A	IFNg	CD5	CD23	CD23	CD5	CD5	Lineage^*	CD13
APC/AF647	CD127	IL-21	IL-2	CD22	IgD	IgD	CD336	CD127	BDCA-2	CD33
AF700	CCR7	CCR7	CCR7	CD11c	IgM	IgM	CD158e1	CD158e1	CD33	CD36
APC-Cy7/AF750	CD3	CD3	CD3	CD25	CD10	CD10	CD3	CD3	TLR-9	CD206

Bold text indicates markers recurring throughout all tubes within a lineage; Italic text indicates intracellular markers requiring permeabilization of the cells.

Lineage: CD3, CD19, CD20, CD14, CD16, CD56