Figure 2.

FRAP at the leading edge of migrating border cells. dmim is an I-BAR protein in Drosophila shown to inhibit endocytosis and regulate the migration of border cells and primordial germ cells.⁶⁷ (A) Photobleaching of an early stage 9 Drosophila border cell clusters expressing UAS-moesin::GFP under 306-Gal4. A 5 µm diameter area was ablated at the leading edge (shown in white brackets) and images were captured at 1 second intervals after photobleaching. Scale bar = 5 µm. (B) Quantitation of FRAP time series shows that while there is only a slight difference in the rate of recovery, there is a significant difference in the mobile fraction of the mutant border cells when compared to wildtype and rescue. Data are represented as the mean ± SEM from 5 separate time series for each genotype. (*p < 0.01, t-test).