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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Peptides. 2010 Jun 15;32(3):595–600. doi: 10.1016/j.peptides.2010.06.005

Figure 4.

Figure 4

Cry4Ba Domain II loop α-8 is involved on Cyt1Aa binding. Different Cry4Ba proteins (three μg) were activated by trypsin treatment separated by 10% SDS-PAGE electrophoresis and blotted into nylon membranes. Blots were revealed with 10 nM Cyt1Aa followed with anti-Cyt1Aa and by a secondary goat-anti-rabbit- antibody. Lane 1 Cry4Ba, lane 2 α8M1 and lane 3 α8M2.